Question 23M.3.HL.TZ1.11
Date | May 2023 | Marks available | [Maximum mark: 6] | Reference code | 23M.3.HL.TZ1.11 |
Level | HL | Paper | 3 | Time zone | TZ1 |
Command term | Describe, Explain, State | Question number | 11 | Adapted from | N/A |
The image shows the life cycle of a biofilm.
[Source: Tang, L., Zhang, Y., Li, C., et al., 2022. Biological Stability of Water-Based Cutting Fluids: Progress and Application.
Chin. J. Mech. Eng. [e-journal] 35(3). https://doi.org/10.1186/s10033-021-00667-z. Open access.]
State one function of biopolymers (EPS) in the biofilm.
[1]
allows bacteria to stick together/to the surface/retains water/protection/structural stability;
This was often the only part of question 11 which candidates were able to answer correctly. This may have been because the diagram provided showed EPS and 'irreversible attachment".

Describe emergent properties in mature colonies of biofilms, other than EPS production.
[2]
- emergent properties are those in the biofilm that may not be present in individual cells/are outcomes of interactions of the elements of a system;
- resistance to antibiotics due to reduced cell division rates;
OR
resistance to antimicrobials due to physical barriers to penetration; - social cooperation as cells organize to form a complex structure
OR
release of signaling molecules leads to gene expression to develop biofilm; - cells use matrix to move, leading to (whole) colony moving;
- formation of channels for water flow inside a colony;
Accept other valid examples with descriptions
Few could describe emergent properties in mature colonies of biofilms, many simply repeating what was already given in part (a).

Explain how biofilms can be useful in the treatment of sewage.
[3]
- (trickling filter) system/bed uses support medium/rocks/ stones/rubber/plastic on which biofilm develops/ grows;
- sewage trickled/sprinkled/dripped/sprayed over the medium to provideaeration/oxygen;
- biofilms feed off/digest organic matter in the sewage to obtain energy/forgrowth;
- resulting sludge removed may be used as fertilizer;
Very few understood the role of biofilms in sewage treatment.
