Question 23M.3.SL.TZ2.2
Date | May 2023 | Marks available | [Maximum mark: 5] | Reference code | 23M.3.SL.TZ2.2 |
Level | SL | Paper | 3 | Time zone | TZ2 |
Command term | Explain, Identify, Outline | Question number | 2 | Adapted from | N/A |
Outline the effect of low temperature on enzyme activity.
[1]
slows down/inactivates the enzyme;
Parts (a) of this question on enzymes was well answered.

Guppies (Poecilia reticulata), popular aquarium fish, were used to test the effect of changes in pH on the activity of intestinal protease enzymes in each sex.
[Source: Thongprajukaew, K. and Kovitvadhi, U., 2013. Effects of sex on characteristics and expression
levels of digestive enzymes in the adult guppy Poecilia reticulata. Zoological Studies 52.
https://zoologicalstudies.springeropen.com/articles/10.1186/1810-522X-52-3. Open access.]
Identify the independent and dependent variables in this study.
Independent variable:
Dependent variable:
[2]
- Independent variable: pH / sex;
- Dependent variable: enzyme/protease activity;
Parts (b) of this question on enzymes was well answered.
Explain how change in pH affects the protease activity of the females.
[2]
- change in acidity/pH changes the charges/shape/denatures/structure of the enzyme;
- changes may alter the ability of the enzyme to bind / binding of substrate;
- greatest/peak/maximum activity is at optimal pH/pH10;
Part (c) was not well answered with most candidates only describing the shape of the graph when an explanation for the change of shape was required. Many candidates scored 1 mark for stating that the optimum or peak pH was at pH 10.
