DP Biology (last assessment 2024)

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Question 22M.2.SL.TZ2.5b

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Date May 2022 Marks available [Maximum mark: 2] Reference code 22M.2.SL.TZ2.5b
Level SL Paper 2 Time zone TZ2
Command term Outline Question number b Adapted from N/A
b.
[Maximum mark: 2]
22M.2.SL.TZ2.5b

Enzyme activity is affected by temperature and pH.

(b)

In biotechnology, enzymes are used to transfer genes to bacteria. Outline how two specific enzymes are used for the transfer.

[2]

Markscheme
  1. restriction enzymes/(restriction) endonucleases cut the gene and the bacterial/plasmid/vector DNA in the same/specific restriction sites
    OR
    (restriction) endonucleases work by targeting a specific sequence of base pairs in DNA causing both strands of the DNA to break apart;
  2. (DNA) ligase attaches/inserts the gene to the bacterial/plasmid/vector DNA
    OR
    (DNA) ligase joins the vector and gene by fusing their sugar-phosphate backbones together (with a covalent phosphodiester bond);
  3. correct reference to reverse transcriptase;

Accept correct mention of reverse transcriptase.

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