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Question 19M.3.HL.TZ1.9a.iii

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Date May 2019 Marks available [Maximum mark: 1] Reference code 19M.3.HL.TZ1.9a.iii
Level HL Paper 3 Time zone TZ1
Command term Suggest Question number a.iii Adapted from N/A
a.iii.
[Maximum mark: 1]
19M.3.HL.TZ1.9a.iii

Discs were soaked in different antibiotics and were placed on a culture of Bacillus subtilis spread on sterile agar in a Petri dish. The Petri dish was left in an incubator, after which zones of inhibition were observed surrounding some of the discs. The photograph is to scale.

[Source: Tasha L. Sturm. 2009. Kirby-Bauer disk diffusion susceptibility test. Visual Resources. American Society for
Microbiology, Washington, DC. www.microbelibrary.org Accessed 29 September 2014]

(a.iii)

Suggest a reason for the result with disc X.

[1]

Markscheme

a. it contained no antibiotic ✔

b. it was a control «with only water» ✔ 

c. the antibiotic it contained was not effective against the bacterium/bacterium is resistant to antibiotic ✔

Examiners report

This question was also well answered, with most students recognising the disc as a control or the fact that the bacteria were resistant. Weaker students struggled with subject-specific terminology and mistakenly used terms such as “antibodies” (instead of “antibiotics”) and “immune” (instead of “resistant”).