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Question 19M.3.HL.TZ1.11

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Date May 2019 Marks available [Maximum mark: 4] Reference code 19M.3.HL.TZ1.11
Level HL Paper 3 Time zone TZ1
Command term Identify, Outline, State, Suggest Question number 11 Adapted from N/A
11.
[Maximum mark: 4]
19M.3.HL.TZ1.11

Some halophilic bacteria biodegrade benzene, which makes them useful in treating oil spills as benzene is contained in crude oil. Investigators added cultures of the bacteria to benzene solutions at different salinities in containers. The amount of benzene remaining in the container was recorded once a week for two weeks.

[Source: adapted from C A Nicholson and B Z Fathepure, (2004),
Applied and Environmental Microbiology, 70 (2), pages 1222–1225]

(a)

Outline what is meant by halophilic when describing the bacteria.

[1]

Markscheme

thrive/live in a salt rich environment ✔

Examiners report

(a) to (c) These three questions were often answered well.

(b)

Identify the salt concentration with the greatest rate of benzene biodegradation.

………………......… mol m–3

[1]

Markscheme

1 mol m–3

Examiners report

(a) to (c) These three questions were often answered well.

(c)

State one genus of halophilic bacteria known to biodegrade benzene.

[1]

Markscheme

Marinobacter ✔

Allow other correct responses eg: Dechloromonas, Haloferax, Pseudomonas, Fusarium, Halomonas, Halococcus,
Halobacterium, Haloarcula, Haloarchaea, Fundibacter, Fusarium, Alcanivorax, Dietzia

Examiners report

(a) to (c) These three questions were often answered well.

(d)

Suggest one advantage to the bacteria of breaking down benzene.

[1]

Markscheme

a. energy gained from the benzene ✔ 

b. benzene acts as a source of carbon ✔

Examiners report

Very few students could state that the breakdown of benzene could be used as a source of carbon or energy.