DP Biology (first assessment 2025)

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Question SPM.1B.SL.TZ0.2

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Date May Specimen-2023 Marks available [Maximum mark: 7] Reference code SPM.1B.SL.TZ0.2
Level SL Paper 1B Time zone TZ0
Command term Calculate, Draw, Identify, Suggest Question number 2 Adapted from N/A
2.
[Maximum mark: 7]
SPM.1B.SL.TZ0.2

The micrograph shows a transverse section of a leaf from a flowering plant.

[Source: Houseman, J. and Ford, M., 2014. Dicot leaf L. [image online] Available at: https://commons.wikimedia.org/wiki/
File:Dicot_leaf_L.jpg Distributed under the terms of the Creative Commons Attribution License 4.0 (CC BY-SA 4.0). https://creativecommons.org/licenses/by-sa/4.0/deed.en Image cropped and re-coloured.]

(a)

Draw a plan diagram in the right-hand box with labels to show the distribution of tissues in this transverse section of a leaf.

[3]

Markscheme
  1.  palisade mesophyll;
  2.  spongy mesophyll;
  3.  lower epidermis;
  4.  upper epidermis;
  5.  xylem/phloem/vascular bundle;

1 mark for each correctly labelled tissue shown as a layer in proportion.

(b)

Calculate the actual thickness of the leaf, from upper to lower surface along the line a–b, showing your working.

[2]

Markscheme
  1.  correct measurement of the line on the image (+/-1mm);
  2.  correct calculation and including units;

Allow ECF – ruler measurements +/- 1mm

(c)

Suggest a reason for using a lower power objective lens when first focusing on a slide under the microscope.

[1]

Markscheme
  1.   it is easier to find the specimen using a low power objective lens
      OR
      there is a larger field of view/depth of focus;
  2.   it is easier to locate the most interesting part of the specimen with a low power objective lens;
  3.   most microscopes are calibrated so that once in focus using the low power objective lens they will be close    to focus using the higher power, so this helps focusing;
  4.   reduced risk of cracking the slide;
(d)

Identify one adaptation of the leaf for the absorption of light visible in this micrograph.

[1]

Markscheme
  1.  double layer of palisade cells;
  2.  palisade layer on upper surface;
  3.  dimensions of palisade cells maximize light absorption;
  4.  thin/transparent epidermis/cuticle;