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Question 18M.3.HL.TZ2.d

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Date May 2018 Marks available [Maximum mark: 2] Reference code 18M.3.HL.TZ2.d
Level HL Paper 3 Time zone TZ2
Command term List Question number d Adapted from N/A
d.
[Maximum mark: 2]
18M.3.HL.TZ2.d

A protein expressed by the gene carotene desaturase (CTP1) was introduced into Golden rice (Oryza sativa) through genetic engineering. Bioinformatics were used to determine if the protein sequence matched any allergenic proteins (proteins that trigger an allergic reaction). The table shows the results from the alignment of the CTP1 protein with proteins from moth, soybean and dust mite that are known to be allergenic.

Physical and chemical methods can be used to genetically modify crop plants by inserting new genes. List a physical and a chemical method that could have been used to introduce the gene coding for CTP1 into the rice plants.

 

[2]

Markscheme

a. physical:
electroporation
OR
microinjection
OR
biolistics «gunshot» 

Allow other verifiable answer.

b. chemical:
calcium chloride
OR
liposomes

Allow other verifiable answer.