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Question 19M.3.SL.TZ2.a

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Date May 2019 Marks available [Maximum mark: 2] Reference code 19M.3.SL.TZ2.a
Level SL Paper 3 Time zone TZ2
Command term Outline Question number a Adapted from N/A
a.
[Maximum mark: 2]
19M.3.SL.TZ2.a

The diagram shows a method of production of transgenic plants and some of their uses.

[Source: Jian Yao, et al., (2015), International Journal of Molecular Science, 2015, 16(12),
28549–28565; https://doi.org/10.3390/ijms161226122]

Outline how the target gene is found using bioinformatics.

[2]

Markscheme

a. open reading frame finder «ORF finder» detects sections of a DNA molecule likely to be genes
OR
search for significant length of DNA coding from a start codon to a stop codon ✔

b. BLASTn used to compare gene sequences coding for similar proteins in other organisms/databases ✔

c. BLASTp used to detect similar proteins in other organisms/databases ✔

Accept methionine/AUG as start codon and UAA/UAG/UGA as stop codon.

Accept any other verified software program.

Examiners report

The question showed a diagram of the production of transgenic plants. Parts (a) and (c) proved difficult due to lack of specific vocabulary.