Question 19M.3.SL.TZ2.9a
| Date | May 2019 | Marks available | [Maximum mark: 2] | Reference code | 19M.3.SL.TZ2.9a |
| Level | SL | Paper | 3 | Time zone | TZ2 |
| Command term | Outline | Question number | a | Adapted from | N/A |
The diagram shows a method of production of transgenic plants and some of their uses.
[Source: Jian Yao, et al., (2015), International Journal of Molecular Science, 2015, 16(12),
28549–28565; https://doi.org/10.3390/ijms161226122]
Outline how the target gene is found using bioinformatics.
[2]
a. open reading frame finder «ORF finder» detects sections of a DNA molecule likely to be genes
OR
search for significant length of DNA coding from a start codon to a stop codon ✔
b. BLASTn used to compare gene sequences coding for similar proteins in other organisms/databases ✔
c. BLASTp used to detect similar proteins in other organisms/databases ✔
Accept methionine/AUG as start codon and UAA/UAG/UGA as stop codon.
Accept any other verified software program.
The question showed a diagram of the production of transgenic plants. Parts (a) and (c) proved difficult due to lack of specific vocabulary.
