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Question 19M.3.HL.TZ2.11

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Date May 2019 Marks available [Maximum mark: 5] Reference code 19M.3.HL.TZ2.11
Level HL Paper 3 Time zone TZ2
Command term Determine, Outline Question number 11 Adapted from N/A
11.
[Maximum mark: 5]
19M.3.HL.TZ2.11
(a)

A segment of DNA is shown. Determine a possible open reading frame (ORF) segment in the DNA segment by completing the table.

[2]

Markscheme

a. correct starting point ✔
eg

OR

b. correct RNA nucleotides ✔

Examiners report

Most candidates had difficulty completing the diagram and/or used DNA nucleotides instead of RNA nucleotides.

(b.i)

In gene research, outline the use of open reading frames.

[1]

Markscheme

identify a sequence/gene «that has the potential to be transcribed» ✔

Examiners report

Knowledge of different tools in gene research seems to be fine, but gene knockout was less understood.

(b.ii)

In gene research, outline the use of gene knockout.

[1]

Markscheme

silence gene to observe the effect when the gene is not expressed
OR
change in phenotype to deduce function of gene ✔

Examiners report

Knowledge of different tools in gene research seems to be fine, but gene knockout was less understood.

(b.iii)

In gene research, outline the use of BLASTn.

[1]

Markscheme

to compare nucleotide/DNA sequence with other «nucleotide» sequences ✔